Cardiovascular and Metabolic Disease Center
Mitochondrial Research Affinity Collaboration-Laboratories & Engineering

Home > 0

와우!! 축하합니다. 염재범 선생님 논문 2편이 출판되었습니다.

  • 작성자한진
  • 작성일2006-04-14 02:02:29
  • 조회수2583
  • 첨부파일첨부파일
Philos Transact A Math Phys Eng Sci. 2006 May 15;364(1842):1135-54. A mathematical model of pacemaker activity recorded from mouse small intestine. Youm JB, Kim N, Han J, Kim E, Joo H, Leem CH, Goto G, Noma A, Earm YE. College of Medicine, 2020 Cardiovascular Institute, Inje University Mitochondrial Signaling Laboratory, Department of Physiology and Biophysics Busan 614-735, South Korea. The pacemaker activity of interstitial cells of Cajal (ICCs) has been known to initiate the propagation of slow waves along the whole gastrointestinal tract through spontaneous and repetitive generation of action potentials. We studied the mechanism of the pacemaker activity of ICCs in the mouse small intestine and tested it using a mathematical model. The model includes ion channels, exchanger, pumps and intracellular machinery for Ca(2+) regulation. The model also incorporates inositol 1,4,5-triphosphate (IP(3)) production and IP(3)-mediated Ca(2+) release activities. Most of the parameters were obtained from the literature and were modified to fit the experimental results of ICCs from mouse small intestine. We were then able to compose a mathematical model that simulates the pacemaker activity of ICCs. The model generates pacemaker potentials regularly and repetitively as long as the simulation continues. The frequency was set at 20min(-1) and the duration at 50% repolarization was 639ms. The resting and overshoot potentials were -78 and +1.2mV, respectively. The reconstructed pacemaker potentials closely matched those obtained from animal experiments. The model supports the idea that cyclic changes in [Ca(2+)](i) and [IP(3)] play key roles in the generation of ICC pacemaker activity in the mouse small intestine. ================================================================================================ 1: Philos Transact A Math Phys Eng Sci. 2006 May 15;364(1842):1223-43. Links Simulation of Ca(2+)-activated Cl(-) current of cardiomyocytes in rabbit pulmonary vein: implications of subsarcolemmal Ca(2+) dynamics. Leem CH, Kim WT, Ha JM, Lee YJ, Seong HC, Choe H, Jang YJ, Youm JB, Earm YE. University of Ulsan College of Medicine Department of Physiology 388-1 Poongnap-Dong Songpa-Ku, Seoul 138-736, South Korea. In recent studies, we recorded transiently activated outward currents by the application of three-step voltage pulses to induce a reverse mode of Na(+)-Ca(2+) exchange (NCX). We found that these currents were mediated by a Ca(2+)-activated Cl(-) current. Based on the recent reports describing the atrial Ca(2+) transients, the Ca(2+) transient at the subsarcolemmal space was initiated and then diffused into the cytosolic space. Because the myocardium in the pulmonary vein is an extension of the atrium, the Ca(2+)-activated Cl(-) current may reflect the subsarcolemmal Ca(2+) dynamics. We tried to predict the subsarcolemmal Ca(2+) dynamics by simulating these current traces. According to recent reports on the geometry of atrial myocytes, we assumed that there were three compartments of sarcoplasmic reticulum (SR): a network SR, a junctional SR and a central SR. Based on these structures, we also divided the cytosolic space into three compartments: the junctional, subsarcolemmal and cytosolic spaces. Geometry information and cellular capacitance suggested that there were essentially no T-tubules in these cells. The basic physical data, such as the compartmental volumes, the diffusion coefficients and the stability coefficients of the Ca(2+) buffers, were obtained from the literature. In the simulation, we incorporated the NCX, the L-type Ca(2+) channel, the rapid activating outward rectifier K(+) channel, the Na(+)-K(+) pump, the SR Ca(2+)-pump, the ryanodine receptor, the Ca(2+)-activated Cl(-) channel and the dynamics of Na(+), K(+), Ca(2+) and Cl(-). In these conditions, we could successfully reconstruct the Ca(2+)-activated Cl(-) currents. The simulation allowed estimation of the Ca(2+) dynamics of each compartment and the distribution of the Ca(2+)-activated Cl(-) channel and the NCX in the sarcolemma on the junctional or subsarcolemmal space.
Total404 [ page1/27 ]
No. 제목 작성자 작성일 조회수
404 인제대, 과기정통부 ‘2023년도 기초연구실 지원사업’ 선정 2023.09.13 관리자 (web_admin) 2023.09.13 7
403 인제대 교수팀 '돌연사 주범 심부전 원인 규명' 2023.09.13 관리자 (web_admin) 2023.09.13 5
402 2022학년도 인제학술상 수상자 선정 결과 2023.01.05 관리자 2023.01.05 57
401 안전관리 우수연구실 인증 취득 2023.01.05 관리자 2023.01.05 40
400 한진교수 화의자의학상 수상 2023.01.05 관리자 2023.01.05 57
399 이온통로 학회 -Amy 포스터상 수상 2019.01.15 김형규 2019.01.15 2,587
398 센터 겸임교수 조성우 교수 - 한빛사 -JACC Vascular Imaging 2018.12.08 김형규 2018.12.08 2,791
397 2017 IMPACT Symposium 개최 첨부파일 2017.10.31 김보현 2017.10.31 2,588
396 경암바이오유스 2017 첨부파일 2017.08.11 김보현 2017.08.11 2,871
395 KORUS 2017 첨부파일 2017.06.21 김보현 2017.06.21 2,986
394 IMPACT 2016 심포지엄 개최 안내 첨부파일 2016.04.18 관리자 2016.04.18 4,000
393 IMPACT 2015 심포지엄 개최 안내 첨부파일 2015.04.20 서대윤 2015.04.20 2,869
392 2015 중점연구소 성과 전시회 첨부파일 2015.03.31 김형규 2015.03.31 2,462
391 Best Miso Award - 한진 2014.11.04 이정훈 2014.11.04 2,072
390 신진생리학자상 - 김형규 2014.10.29 이정훈 2014.10.29 1,816
처음이전1 2 3 4 5 6 7 8 9 10 다음 마지막