Cardiovascular and Metabolic Disease Center
Mitochondrial Research Affinity Collaboration-Laboratories & Engineering

Home > 0

Mouse Study Shows Gene Therapy Provides Temporary Protection from Radiation

  • 작성자한진
  • 작성일2006-08-13 14:14:45
  • 조회수2415
Radiation radicalizes elements and compounds in the body. Unchecked, these free radicals damage cells, killing them. When enough cells die, the organism succumbs. But a new therapy infused into the cells of mice offers protection against radiation's effects, at least for a short while. Radiation oncologist Joel Greenberger of the University of Pittsburgh and his colleagues have been experimenting with a transgene--manganese superoxide dismutase plasmid liposome (MnSOD-PL)--that has shown the ability to protect cells from radiation in vitro. Tested in mice in specific organs, such as the lungs and bladder, the transgene conferred significant protection and is currently being tested in human lung cancer patients undergoing radiation therapy. The new work shows that mice intravenously infused with the transgene enjoy protection throughout their bodies when exposed to radiation at levels known to kill 50 percent of their normal peers. ADVERTISEMENT (article continues below) "These are experiments in which mice are given total body radiation, simulating what would happen in the event of exposure to a fission bomb or a dirty bomb with significant inhalation or ingestion of radiation," Greenberger explains. "That's what's known as the LD 50/30: lethal to 50 percent of the mice in 30 days. With this gene therapy, instead of 50 percent dying, we have about 80 percent surviving." The transgene seems to work by inserting itself into the mitochondria. Once there, it convinces the cell to overproduce MnSOD, which apparently repels the damaging effects of radiation-produced free radicals. "The material is getting into the cells and it's just buffering or preventing the depletion of the antioxidant pool," Greenberger says. "It gives cells, tissues and organs time to repair themselves." The effect is transient; the body flushes the transgene within 72 hours unless more is administered. And it is that very transience that seems to confer the protection; mice bred to overproduce MnSOD have no greater ability to survive radiation for reasons that remain unclear. The effect has withstood repeated tests and seems to shield the body, tissue, organ or cell for the duration of its persistence in the mitochondria. "The protection is from the inside out," Greenberger notes. "This is something that would be given to first responders." His team is now searching for a smaller molecule with similar effects that could be delivered in a pill or via a skin patch. Greenberger presented the finding on June 3 at a meeting of the American Society of Gene Therapy.
Total405 [ page10/27 ]
No. 제목 작성자 작성일 조회수
270 Congratulation Mr Kim 2007.05.25 박원선 2007.05.25 2,217
269 축하 드립니다! 김형규샘! 2007.05.17 강성현 2007.05.17 2,717
268 고재홍선생님 좋아하는 HIF-1이네요. 2007.05.13 한진 2007.05.13 3,250
267 Congratulation Dr Park - for new paper 2007.04.30 강성현 2007.04.30 3,640
266 HUPO 6th Annual World Congress, Seoul 2007 첨부파일 2007.04.17 한진 2007.04.17 2,219
265 Congratulation, Dr. Park! 2007.04.12 한진 2007.04.12 2,049
264 당뇨병, 세계를 위협하는 무서운 질병으로 발전 2007.04.08 한진 2007.04.08 2,123
263 Defective mitochondrial biogenesis: a hallmark of the high cardiovascular risk in the metabolic syndrome? 2007.04.05 한진 2007.04.05 2,963
262 Congratulation to Prof. Warda! 2007.04.04 한진 2007.04.04 2,302
261 Congratulation!! Cuong 2007.03.27 박원선 2007.03.27 2,016
260 Patch setting및 mito puller setting 2007.03.19 박원선 2007.03.19 2,326
259 2006 HUPO 에서 뽑은 각 분야 포스터에 저희 포스터가 4개 선정되었습니다. 2007.03.17 김형규 2007.03.17 3,719
258 Two NIH funded postdoctoral positions are available immediately 2007.03.09 한진 2007.03.09 2,282
257 Please share the attached paper with all lab members. 첨부파일 2007.02.24 한진 2007.02.24 1,959
256 Congratulations to Sunghyun Kang 2007.02.22 한진 2007.02.22 1,922
처음이전 1 2 3 4 5 6 7 8 9 10 다음 마지막