Cardiovascular and Metabolic Disease Center
Mitochondrial Research Affinity Collaboration-Laboratories & Engineering

Home > 0

Mouse Study Shows Gene Therapy Provides Temporary Protection from Radiation

  • 작성자한진
  • 작성일2006-08-13 14:14:45
  • 조회수2415
Radiation radicalizes elements and compounds in the body. Unchecked, these free radicals damage cells, killing them. When enough cells die, the organism succumbs. But a new therapy infused into the cells of mice offers protection against radiation's effects, at least for a short while. Radiation oncologist Joel Greenberger of the University of Pittsburgh and his colleagues have been experimenting with a transgene--manganese superoxide dismutase plasmid liposome (MnSOD-PL)--that has shown the ability to protect cells from radiation in vitro. Tested in mice in specific organs, such as the lungs and bladder, the transgene conferred significant protection and is currently being tested in human lung cancer patients undergoing radiation therapy. The new work shows that mice intravenously infused with the transgene enjoy protection throughout their bodies when exposed to radiation at levels known to kill 50 percent of their normal peers. ADVERTISEMENT (article continues below) "These are experiments in which mice are given total body radiation, simulating what would happen in the event of exposure to a fission bomb or a dirty bomb with significant inhalation or ingestion of radiation," Greenberger explains. "That's what's known as the LD 50/30: lethal to 50 percent of the mice in 30 days. With this gene therapy, instead of 50 percent dying, we have about 80 percent surviving." The transgene seems to work by inserting itself into the mitochondria. Once there, it convinces the cell to overproduce MnSOD, which apparently repels the damaging effects of radiation-produced free radicals. "The material is getting into the cells and it's just buffering or preventing the depletion of the antioxidant pool," Greenberger says. "It gives cells, tissues and organs time to repair themselves." The effect is transient; the body flushes the transgene within 72 hours unless more is administered. And it is that very transience that seems to confer the protection; mice bred to overproduce MnSOD have no greater ability to survive radiation for reasons that remain unclear. The effect has withstood repeated tests and seems to shield the body, tissue, organ or cell for the duration of its persistence in the mitochondria. "The protection is from the inside out," Greenberger notes. "This is something that would be given to first responders." His team is now searching for a smaller molecule with similar effects that could be delivered in a pill or via a skin patch. Greenberger presented the finding on June 3 at a meeting of the American Society of Gene Therapy.
Total405 [ page3/27 ]
No. 제목 작성자 작성일 조회수
375 Error bars in experimental biology 첨부파일 2011.05.02 허혜진 2011.05.02 3,128
374 파킨슨 질병은 미토콘드리아 complex 1 손상 보다 microtuble 에 의해서 생길수도... 라는 논문 2011.03.08 김형규 2011.03.08 5,383
373 운동을 안해도,운동한 것처럼 건강한 심장을 만들어 주는 유전자 2011.01.06 서대윤 2011.01.06 3,899
372 2010 ㅡmitophysiology conference, Young Investigator Award 수상 2010.11.16 김형규 2010.11.16 3,353
371 3차원 세포의 세계 2010.10.27 김민희 2010.10.27 2,641
370 인제대 '심혈관.대사질환센터' 중점연구소 선정 -연합뉴스 2010.08.10 김형규 2010.08.10 4,078
369 7월 연구노트 작성 우수상 시상 2010.08.03 김민희 2010.08.03 2,992
368 NYAS 리뷰 논문 게재 - 김형규,하승희 2010.08.02 김형규 2010.08.02 3,723
367 박원선 교수님과 최일환 교수님의 논문이 publish 되었습니다. 2010.07.09 최성우 2010.07.09 4,350
366 김형규-부티투 4회 미토콘드리아 연구의학회 best research award 수상 첨부파일 2010.07.06 김형규 2010.07.06 2,189
365 6월 연구노트 작성 우수상 시상 2010.06.28 최성우 2010.06.28 2,544
364 4th Conference of Korean Society for Mitochondrial Reaserch and Medicine 첨부파일 2010.06.17 최성우 2010.06.17 3,672
363 5월 연구노트 작성 우수상 시상 2010.05.31 최성우 2010.05.31 3,002
362 안준석 - 제18회 기초의학 학술대회,학생논문발표대회 최우수상 수상 첨부파일 2010.05.28 최성우 2010.05.28 3,377
361 제 6회 논문연구계획서 발표대회: 최성우 학생 우수상 수상 첨부파일 2010.04.28 최성우 2010.04.28 2,859
처음이전 1 2 3 4 5 6 7 8 9 10 다음 마지막