Most accessed articles in 2006 Please look the 18th article. 1. Marc R. Wilkins, Ron D. Appel, Jennifer E. Van Eyk, Maxey C. M. Chung, Angelika Görg, Michael Hecker, Lukas A. Huber, Hanno Langen, Andrew J. Link, Young-Ki Paik, Scott D. Patterson, Stephen R. Pennington, Thierry Rabilloud, Richard J. Simpson, Walter Weiss, Michael J. Dunn Guidelines for the next 10 years of proteomics [Viewpoint] (vol. 6, no. 1) 2. Angelika Görg, Walter Weiss, Michael J. Dunn Current two-dimensional electrophoresis technology for proteomics [Review] (vol. 4, no. 12) 3. Joerg Reinders, Albert Sickmann State-of-the-art in phosphoproteomics [Review] (vol. 5, no. 16) 4. Sandra Morandell, Taras Stasyk, Karin Grosstessner-Hain, Elisabeth Roitinger, Karl Mechtler, Guenther K. Bonn, Lukas A. Huber Phosphoproteomics strategies for the functional analysis of signal transduction [Review] (vol. 6, no. 14) 5. Gilbert S. Omenn, David J. States, Marcin Adamski, Thomas W. Blackwell, Rajasree Menon, Henning Hermjakob, Rolf Apweiler, Brian B. Haab, Richard J. Simpson, James S. Eddes, Eugene A. Kapp, Robert L. Moritz, Daniel W. Chan, Alex J. Rai, Arie Admon, Ruedi Aebersold, Jimmy Eng, William S. Hancock, Stanley A. Hefta, Helmut Meyer, Young-Ki Paik, Jong-Shin Yoo, Peipei Ping, Joel Pounds, Joshua Adkins, Xiaohong Qian, Rong Wang, Valerie Wasinger, Chi Yue Wu, Xiaohang Zhao, Rong Zeng, Alexander Archakov, Akira Tsugita, Ilan Beer, Akhilesh Pandey, Michael Pisano, Philip Andrews, Harald Tammen, David W. Speicher, Samir M. Hanash Overview of the HUPO Plasma Proteome Project: Results from the pilot phase with 35 collaborating laboratories and multiple analytical groups, generating a core dataset of 3020 proteins and a publicly-available database [Regular Article] (vol. 5, no. 13) 6. Yuri D. Ivanov, Vadim M. Govorun, Victor A. Bykov, Alexander I. Archakov Nanotechnologies in proteomics [Review] (vol. 6, no. 5) 7. Robert Drakas, Marco Prisco, Renato Baserga A modified tandem affinity purification tag technique for the purification of protein complexes in mammalian cells [Regular Article] (vol. 5, no. 1) 8. Lynne Thadikkaran, Michèle A. Siegenthaler, David Crettaz, Pierre-Alain Queloz, Philippe Schneider, Jean-Daniel Tissot Recent advances in blood-related proteomics [Review] (vol. 5, no. 12) 9. Katherine Hollywood, Daniel R. Brison, Royston Goodacre Metabolomics: Current technologies and future trends [Systems Biology] (vol. 6, no. 17) 10. Julian Vasilescu, Xuecui Guo, Juergen Kast Identification of protein-protein interactions using in vivo cross-linking and mass spectrometry [Research Article] (vol. 4, no. 12) 11. Brigitte Wittmann-Liebold, Hanns-Rüdiger Graack, Thomas Pohl Two-dimensional gel electrophoresis as tool for proteomics studies in combination with protein identification by mass spectrometry [Clinical Applications] (vol. 6, no. 17) 12. Tetsuya Okano, Tadashi Kondo, Tatsuhiko Kakisaka, Kiyonaga Fujii, Masayo Yamada, Harubumi Kato, Toshihide Nishimura, Akihiko Gemma, Shoji Kudoh, Setsuo Hirohashi Plasma proteomics of lung cancer by a linkage of multi-dimensional liquid chromatography and two-dimensional difference gel electrophoresis [Clinical Application] (vol. 6, no. 13) 13. J. Douglas Armstrong, Andrew J. Pocklington, Mark A. Cumiskey, Seth G. N. Grant Reconstructing protein complexes: From proteomics to systems biology [Bioinformatics] (vol. 6, no. 17) 14. Willy Morelle, Kévin Canis, Frédéric Chirat, Valegh Faid, Jean-Claude Michalski The use of mass spectrometry for the proteomic analysis of glycosylation [Review] (vol. 6, no. 14) 15. Renate Bublitz, Stefan Kreusch, Günther Ditze, Margarete Schulze, Gerhard A. Cumme, Christine Fischer, Adrienne Winter, Horst Hoppe, Heidrun Rhode Robust protein quantitation in chromatographic fractions using MALDI-MS of tryptic peptides [Clinical Application] (vol. 6, no. 13) 16. Hyun-Kyung Lee, Byung-Hyo Lee, Seung-Ah Park, Chan-Wha Kim The proteomic analysis of an adipocyte differentiated from human mesenchymal stem cells using two-dimensional gel electrophoresis [Research Article] (vol. 6, no. 4) 17. Ingrid Miller, Johanne Crawford, Elisabetta Gianazza Protein stains for proteomic applications: Which, when, why? [Technology] (vol. 6, no. 20) 18. Nari Kim, Youngsuk Lee, Hyungkyu Kim, Hyun Joo, Jae Boum Youm, Won Sun Park, Mohamad Warda, Dang Van Cuong, Jin Han Potential biomarkers for ischemic heart damage identified in mitochondrial proteins by comparative proteomics [Research Article] (vol. 6, no. 4) 19. Serhiy Souchelnytskyi Bridging proteomics and systems biology: What are the roads to be traveled? [Review] (vol. 5, no. 16) 20. Mikel R. Roe, Timothy J. Griffin Gel-free mass spectrometry-based high throughput proteomics: Tools for studying biological response of proteins and proteomes [Technology] (vol. 6, no. 17) 21. Raimund Hoffrogge, Stefan Mikkat, Christian Scharf, Susanne Beyer, Hilmar Christoph, Jens Pahnke, Eilhard Mix, Matthias Berth, Adelinde Uhrmacher, Igor Z. Zubrzycki, Erik Miljan, Uwe Völker, Arndt Rolfs 2-DE proteome analysis of a proliferating and differentiating human neuronal stem cell line (ReNcell VM) [Research Article] (vol. 6, no. 6) 22. John M. Luk, Chi-Tat Lam, Annie F. M. Siu, Brian Y. Lam, Irene O. L. Ng, Mei-Yu Hu, Chi-Ming Che, Sheung-Tat Fan Proteomic profiling of hepatocellular carcinoma in Chinese cohort reveals heat-shock proteins (Hsp27, Hsp70, GRP78) up-regulation and their associated prognostic values [Research Article] (vol. 6, no. 3) 23. Lynn A. Echan, Hsin-Yao Tang, Nadeem Ali-Khan, KiBeom Lee, David W. Speicher Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma [Regular Article] (vol. 5, no. 13) 24. Hossein Baharvand, Mohsen Hajheidari, Saeid Kazemi Ashtiani, Ghasem Hosseini Salekdeh Proteomic signature of human embryonic stem cells [Short Communication] (vol. 6, no. 12) 25. Roberto Raggiaschi, Chiara Lorenzetto, Enrica Diodato, Andrea Caricasole, Stefano Gotta, Georg C. Terstappen Detection of phosphorylation patterns in rat cortical neurons by combining phosphatase treatment and DIGE technology [Research Article] (vol. 6, no. 3) 26. Ping Yang, Heidi M. Sampson, Henry M. Krause A modified tandem affinity purification strategy identifies cofactors of the Drosophila nuclear receptor dHNF4 [Research Article] (vol. 6, no. 3)